| 紫外分光光度法测定白芍总苷的含量 |
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| 引用本文： | 吴英华,任凤莲,蔡小华,季怀萍,瞿白露.紫外分光光度法测定白芍总苷的含量[J].应用化工,2007,36(8):822-824. |
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| 作者姓名： | 吴英华 任凤莲 蔡小华 季怀萍 瞿白露 |
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| 作者单位： | 1. 怀化医学高等专科学校,公共课部,湖南,怀化,418000
2. 中南大学,化学化工学院,湖南,长沙,410083 |
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| 摘 要： | 研究了白芍总皂苷的紫外吸收特性,建立了以苯甲酸为对照品,用紫外分光光度法测定白芍总皂苷含量的方法。方法的回归方程为:Y=0.003 24+0.107 63c,相关系数0.998 85,加标回收率为95.7%~102.0%,RSD=2.57%(n=9)。方法简便易行、快速、准确。
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| 关 键 词： | 白芍 白芍总苷 苯甲酸 分光光度法 |
| 文章编号： | 1671-3206（2007）08-0822-03 |
| 修稿时间： | 2007-05-22 |
Determination of paeoniflorin in Paeonia lactiflora Pall by UV spectrophotometry |
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| WU Ying-hua,REN Feng-lian,CAI Xiao-hua,JI Huai-ping,QU Bai-lu.Determination of paeoniflorin in Paeonia lactiflora Pall by UV spectrophotometry[J].Applied chemical industry,2007,36(8):822-824. |
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| Authors: | WU Ying-hua REN Feng-lian CAI Xiao-hua JI Huai-ping QU Bai-lu |
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| Affiliation: | 1. The General Course Department, Huaihua Medical College, Huaihua 418000, China ;2. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China |
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| Abstract: | The UV-absorption characteristics of the paeoniflorin in Paeonia lactiflora Pall were discussed,and the UV-absorption method for determination of total paeoniflorin with benzoic acid as control was developed.The regression equation was Y=0.003 24+0.107 63 c with a correlation coefficient of 0.998 85.The standard addition recovery of elements was 95.7%～102.0% and RSD was 2.57%(n=9).This method was simple,fast and accurate. |
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| Keywords: | Paeonia lactiflora Pall paeoniflorin benzoic acid UV spectrophotometry |
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