基于微流控技术的体外多参数快速诊断POCT系统的设计

针对传统的体外快速诊断系统(POCT)精度低、参数单一等问题,设计了一种基于微纳传感结合微流控技术的多参数体外快速诊断POCT系统.本文研究了传感器与微流控芯片的制备工艺、传感器微弱信号的采集与提取、多通道参考电极的多维度抗干扰校正算法以及多参数POCT系统的优化设计,保证了POCT系统具有检测精度高、抗干扰性能好、多参数集成等特点.通过系统测试和临床实际样本的基本性能测试,实验结果表明,本文提出的多参数体外诊断快速检测POCT系统能够综合检测血糖、血酮等多项指标,具备快速,精确,多参数测量的特点,将为基层医疗机构的生化指标检测提供一种新型实用的技术和仪器.

Multi-Parameter Point of Care Testing System Based on Micro-nano Sensors and Microfluidics Technology

In order to solve the problems of low precision and single parameter in the traditional in-vitro rapid diag-nostic system,a high precision and multi-parameter rapid in-vitro diagnostic system is designed based on the mi-crofluidic and micro-nano sensing technology. The paper mainly includes the introduction and presentation of the following aspects:the microfluidic chip design,the micro-nano sensing preparation and processing technology,the multidimensional anti-interference correction algorithm based on microfluidic multi-channel reference technique,the weak biosensing signal extraction and acquisition,and the multi-parameter in-vitro rapid diagnostic analysis system design. The system has the advantages of high detection accuracy,good anti-jamming performance,and multi-param-eter integration. The basic performance of the multi-parameter rapid detection system is tested using systematic test and clinical blood. The experimental results show that the multi-parameter in-vitro diagnostic rapid detection system can be a comprehensive detection of blood glucose,four items of blood lipids,uric acid,blood ketone and many other indicators,with fast accurate and multi-parameter measurement,which may provide a practical technology platform for the detection of portable biochemical indicators of basic medical institutions.