| 玉米醇溶蛋白水解物分离条件的选择 |
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| 引用本文： | 鞠里,刘波,芦明春,刘英新,金凤燮. 玉米醇溶蛋白水解物分离条件的选择[J]. 大连轻工业学院学报, 2009, 28(1): 17-19 |
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| 作者姓名： | 鞠里 刘波 芦明春 刘英新 金凤燮 |
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| 作者单位： | 大连工业大学生物与食品工程学院; |
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| 基金项目： | 国家自然科学基金资助项目(30371744);;辽宁省教育厅科学研究计划资助项目(2005015) |
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| 摘 要： | 利用Bacillus natto菌所产蛋白酶水解玉米醇溶蛋白,得到水解产物,采用凝胶层析技术,对水解产物的分离条件进行选择。结果表明,Sephadex G-25作为分离介质,流速为1.0 mL/min,上样体积为1.5 mL,在此条件下其分离效果较好,得到5个组分。
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| 关 键 词： | 玉米蛋白 纳豆菌 分离 |
Isolation of hydrolyzing maize-protein on Sephadex G-25 |
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| JU Li,LIU Bo,LU Ming-chun,LIU Ying-xin,JIN Feng-xie School of Biological , Food Engineering,Dalian Polytechnic University,Dalian ,China. Isolation of hydrolyzing maize-protein on Sephadex G-25[J]. Journal of Dalian Institute of Light Industry, 2009, 28(1): 17-19 |
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| Authors: | JU Li LIU Bo LU Ming-chun LIU Ying-xin JIN Feng-xie School of Biological & Food Engineering Dalian Polytechnic University Dalian China |
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| Affiliation: | JU Li,LIU Bo,LU Ming-chun,LIU Ying-xin,JIN Feng-xie School of Biological & Food Engineering,Dalian Polytechnic University,Dalian 116034,China |
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| Abstract: | The ethanol-soluble proteins of corn were hydrolyzed by proteases that were produced by solid fermentation with Bacillus natto.The separation of hydrolysates was examined and five components were obtain when separated on Sephadex G-25 at flow rate 1.0 mL/min,and amount of sample was 1.5 mL. |
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| Keywords: | zein Bacillus natto separation |
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