Dietary fats and properties of endoplasmic reticulum: II. Dietary lipid induced changes in activities of drug metabolizing enzymes in liver and duodenum of rat |
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Authors: | E Hietanen M Laitinen H Vainio O Hänninen |
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Affiliation: | (1) Department of Physiology, University of Kuopio, SF-70100 Kuopio, Finland |
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Abstract: | Rats were fed cholesterol, cacao butter, or olive oil diets to determine the effect of dietary lipids on the rate of drug
biotransformation in the liver and duodenum. The cholesterol rich diet maintained the hepatic aryl hydrocarbon hydroxylase
activity at the same level as did the standard diet. Rats fed olive oil and cacao butter diets showed lower hepatic aryl hydrocarbon
hydrorylase activity. The p-nitroanisole 0-demethylase activity was doubled in hepatic microsomes of rats fed the high cholesterol
diet when compared to rats fed the standard diet. The hepatic uridine diphosphate glucoronosyltransferase activity showed
different patterns depending on the in vitro treatment of the microsomal membranes. If the enzyme activity was assayed from
the native, untreated microsomes, an increase in the measurable uridine diphosphate glucuronosyl transferase activity was
found in rats having cholesterol rich diet. After the in vitro activation of membrane-bound uridine diphosphate glucuronosyltransferase
by trypsin, the increase in measurable activity was 10 fold in the group fed the standard diet, 6 fold in group fed cholesterol,
4 fold in group fed cacao butter, and 3 fold in group fed olive oil. Trypsin digestion of microsomes increased the measurable
uridine diphosphate glucuronosyltransferase activity less in rats fed diets rich in neutral fats than those fed the standard
diet. In the duodenal mucosa, lipid diets decreased the activities of drug hydroxylation and glucuronidation. |
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