| Effects of La~（3＋） on osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells |
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| 引用本文： | 张金超,孙静,谷广其,郝晓红,刘丹丹,李亚平,秦新英,王书香.Effects of La~（3＋） on osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells[J].中国稀土学报(英文版),2012,30(1):90-93. |
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| 作者姓名： | 张金超 孙静 谷广其 郝晓红 刘丹丹 李亚平 秦新英 王书香 |
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| 作者单位： | Chemical Biology Key Laboratory of Hebei Province,College of Chemistry and Environmental Science,Hebei University;Affiliated Hospi-tal of Hebei University |
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| 基金项目： | Project supported by the National Natural Science Foundation of China (20971034);Foundation for Key Program of Ministry of Education of China (208018);Returned Scholars of Hebei Province (207041);Natural Science Key Foundation of Hebei Province (B2009000161);Natural Science Foundation of Hebei University |
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| 摘 要： | In order to elucidate the action of La3+ on bone metabolism,effects of La3+ on the osteogenic and adipogenic differentiation of pri-mary mouse bone marrow stromal cells(BMSCs) were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) test,alkaline phosphatase(ALP) activity measurement,mineralized function,oil red O stain and measurement.The results showed that La3+ pro-moted the proliferation of BMSCs except at 1×10-10 and 1×10-6 mol/L.The effect of La3+ on the osteogenic differentiation depended on con-centrations at the 7th day,but the osteogenic differentiation was inhibited at any concentration at the 14th day.La3+ promoted the formation of mineralized matrix nodules except at 1×10-8 and 1×10-5 mol/L.La3+ inhibited adipogenic differentiation except at 1×10-10 and 1×10-7 mol/L at the 10th day,and inhibited adipogenic differentiation except at 1×10-9 mol/L at the 16th day.These findings suggested that La3+ might have protective effect on bone at appropriate dose and time.This would be valuable for better understanding the mechanism of the effect of La3+ on bone metabolism.
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| 关 键 词： | La3+ bone marrow stromal cells osteogenic differentiation adipogenic differentiation mineralization rare earths |
| 收稿时间： | 31 March 2011 |
Effects of La3+ on osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells |
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| ZHANG Jinchao,SUN Jing,GU Guangqi,HAO Xiaohong,LIU Dandan,LI Yaping,QIN Xinying,WANG Shuxiang.Effects of La3+ on osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells[J].Journal of Rare Earths,2012,30(1):90-93. |
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| Authors: | ZHANG Jinchao SUN Jing GU Guangqi HAO Xiaohong LIU Dandan LI Yaping QIN Xinying WANG Shuxiang |
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| Affiliation: | 1(1.Chemical Biology Key Laboratory of Hebei Province,College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China;2.Affiliated Hospi-tal of Hebei University,Baoding 071000,China) |
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| Abstract: | In order to elucidate the action of La3+ on bone metabolism,effects of La3+ on the osteogenic and adipogenic differentiation of pri-mary mouse bone marrow stromal cells(BMSCs) were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) test,alkaline phosphatase(ALP) activity measurement,mineralized function,oil red O stain and measurement.The results showed that La3+ pro-moted the proliferation of BMSCs except at 1×10-10 and 1×10-6 mol/L.The effect of La3+ on the osteogenic differentiation depended on con-centrations at the 7th day,but the osteogenic differentiation was inhibited at any concentration at the 14th day.La3+ promoted the formation of mineralized matrix nodules except at 1×10-8 and 1×10-5 mol/L.La3+ inhibited adipogenic differentiation except at 1×10-10 and 1×10-7 mol/L at the 10th day,and inhibited adipogenic differentiation except at 1×10-9 mol/L at the 16th day.These findings suggested that La3+ might have protective effect on bone at appropriate dose and time.This would be valuable for better understanding the mechanism of the effect of La3+ on bone metabolism. |
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| Keywords: | La3+ bone marrow stromal cells osteogenic differentiation adipogenic differentiation mineralization rare earths |
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