| 来源于酸马奶的植物乳杆菌Bx6-2抑制甜瓜疫霉菌的研究 |
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| 引用本文： | 王海宽,史艳春,张和平,戚薇.来源于酸马奶的植物乳杆菌Bx6-2抑制甜瓜疫霉菌的研究[J].天津轻工业学院学报,2010(1):1-4. |
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| 作者姓名： | 王海宽 史艳春 张和平 戚薇 |
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| 作者单位： | [1]工业微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 [2]乳品生物技术与工程教育部重点实验室,内蒙古农业大学,呼和浩特010018 |
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| 基金项目： | 国家自然科学基金资助项目（20876116;30900961）;乳品生物技术与工程教育部重点实验室开放课题 |
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| 摘 要： | 采用生长速率法测定植物乳杆菌Bx6．2的发酵液对甜瓜疫霉菌菌丝生长的影响，并对抑菌活性物质的理化性质进行了研究．结果表明，该菌的发酵液对甜瓜疫霉菌菌丝的生长有抑制作用．发酵液中的活性物质有较好的热稳定性，在pH6．0时抑菌效果较好，对酸性蛋白酶敏感，对中性蛋白酶不敏感，常温下10d的稳定性较好．通过调节pH、酶处理和加热处理实验，说明其抑菌活性物质不是乳酸，蛋白质类物质起了一定的抑菌作用，但其抑菌现象可能是与其他物质共同作用的结果．
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| 关 键 词： | 植物乳杆菌 甜瓜疫霉菌 植物致病真菌 |
Study on the Inhibition of Phytophthora. drechsleri Tucker by Lactobacillus plantarum Bx6-2 Isolated from Koumiss |
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| WANG Hai-kuan,SHI Yan-chun,ZHANG He-ping,QI Wei.Study on the Inhibition of Phytophthora. drechsleri Tucker by Lactobacillus plantarum Bx6-2 Isolated from Koumiss[J].Journal of Tianjin University of Light Industry,2010(1):1-4. |
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| Authors: | WANG Hai-kuan SHI Yan-chun ZHANG He-ping QI Wei |
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| Affiliation: | 1. Key Laboratory of Industrial Microbiology,Ministry of Education, College of Biotechnology, Tianjin University of Science ＆ Technology,Tianjin 300457, China; 2. Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education, Inner Mongolia Agricultural University,Hohhot 010018,China) |
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| Abstract: | The effect of Lactobacillus plantarum Bx6-2 against the mycelial growth of Phytophthora. drechsleri Tucker was determined by means of growth rate measurements and study on physicochemical characteristics of bioactive metabolite produced by strain Bx6-2. The results show that the culture supernatants of the strains show different degrees of antagonistic activity on the Phytophthora. drechsleri Tucker. The inhibition effect of culture supematant have a good thermal stability,at pH 6.0 the inhibition is good. The inhibition effect are susceptive to acid protease not to neutrase ,room temperature for 10 d respectively. According the analyzes of enzyme treatment,adjusting pH and thermal treatment,the inhibitory substances of Lactobacillus plantarum Bx6-2 are not lactic acid,protein substance act a certain inhibition,but the inhibition phenomenon may be the result of the combined effect of other substances. |
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| Keywords: | Lactobacillus plantarum Phytophthora drechsleri Tucker plantpathogenicfungi |
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