| 牛血清白蛋白分子印迹壳聚糖树脂的制备 |
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| 引用本文： | 高婷,郭敏杰,樊志,郭艳玲.牛血清白蛋白分子印迹壳聚糖树脂的制备[J].天津轻工业学院学报,2010(1):20-23. |
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| 作者姓名： | 高婷 郭敏杰 樊志 郭艳玲 |
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| 作者单位： | 天津科技大学理学院,天津300457 |
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| 基金项目： | 国家自然科学基金资助项目（20704031）;天津市自然科学基金资助项目（09JCYBJC06300） |
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| 摘 要： | 以酰基化的壳聚糖树脂为载体，丙烯酰胺为功能单体，N，N′-亚甲基双丙烯酰胺为交联剂，在模板分子牛血清白蛋白（BSA）存在下，采用直接滴加成球法，制备出对牛血清白蛋白具有特异识别性能的分子印迹聚合物．对BSA的吸附过程进行Langmuir等温吸附模型的数据处理．结果表明，印迹聚合物对模板蛋白的最大吸附量为11．1mg／g，Langmuir等温吸附平衡常数为8．19mL／mg．
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| 关 键 词： | 壳聚糖 牛血清白蛋白 分子印迹 |
Molecular Imprinting Polymer Made of Chitosan for BSA |
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| GAO Ting,GUO Min-jie,FAN Zhi,GUO Yan-ling.Molecular Imprinting Polymer Made of Chitosan for BSA[J].Journal of Tianjin University of Light Industry,2010(1):20-23. |
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| Authors: | GAO Ting GUO Min-jie FAN Zhi GUO Yan-ling |
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| Affiliation: | (College of Science,Tianjin University of Science ＆ Technology,Tianjin 300457 ,China) |
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| Abstract: | Molecularly imprinted polymer bead was prepared by using bovine serum albumin （BSA）as the template, acylated chitosan resin as the supporting matrix, acrylamide as the functional monomer and N,N＇methylenebisacrylamide as crosslinker. The Langmuir isotherm was used to characterize the absorption of BSA,it was shown that the maximum adsorption capacity to template BSA was 11.1 mg/g and Langrnuir adsorption equilibrium constant was 8.19 mL/mg. |
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| Keywords: | chitosan BSA molecular imprinting |
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