| 家蝇蛹凝集素的纯化及对巨噬细胞免疫活性的影响 |
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| 引用本文： | 周明慧,王春玲,赵旭荣,曹小红.家蝇蛹凝集素的纯化及对巨噬细胞免疫活性的影响[J].天津轻工业学院学报,2010(3):5-8. |
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| 作者姓名： | 周明慧 王春玲 赵旭荣 曹小红 |
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| 作者单位： | 食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457 |
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| 基金项目： | 国家自然科学基金资助项目（20676103）;国家高技术研究计划（863计划）基金资助项目（2007AA10Z319） |
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| 摘 要： | 采用缓冲液浸提、亲和层析、超滤和离子交换4种方法，从家蝇蛹中分离纯化出一种D-半乳糖特异性结合的凝集素（MPL-1），该组分具有明显的凝血活性和免疫调节活性．聚丙烯酰胺凝胶电泳和高效液相色谱分析结果表明，MPL-1为单一成分的蛋白，其分子质量为80ku．中性红吞噬实验结果显示，此种凝集素能提高巨噬细胞的吞噬活性，其作用效果具有时间和浓度依赖性．扫描电镜（SEM）观察结果表明，MPL-1作用后的巨噬细胞呈现明显的活化状态，证明MPL-1对巨噬细胞具有明显的免疫调节作用．
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| 关 键 词： | 家蝇蛹 凝集素 纯化 巨噬细胞 免疫调节 |
Purification and Macrophage Immunomodulatory Activity of Lectin from Musca domestica Pupae |
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| ZHOU Ming-hui,WANG Chun-ling,ZHAO Xu-rong,CAO Xiao-hong.Purification and Macrophage Immunomodulatory Activity of Lectin from Musca domestica Pupae[J].Journal of Tianjin University of Light Industry,2010(3):5-8. |
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| Authors: | ZHOU Ming-hui WANG Chun-ling ZHAO Xu-rong CAO Xiao-hong |
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| Affiliation: | (Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science ＆ Technology,Tianjin 300457,China) |
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| Abstract: | A D-galactose specific lectin was purified from Musca domestica pupae （MPL-1）by crude extract in buffer, affinity chromatography,ultra-filtration and ion exchange process, subsequently, lts macrophage immunomodulatory activity in vitro was studied. Detection of PAGE and HPLC show that MPL-1 is homogeneous with the molecular weight of 80 ku. Pinocytic activity of macrophage can be obvious enhanced by MPL-1 in a time and dose dependent manner,and the macrophages are shown to be activated by observation of the microstructures of cells scanning electron microscope （SEM）. |
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| Keywords: | Musca domestica pupae lectin purification immunomodulator macrophage |
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