Molecular analysis of P16(Ink4)/CDKN2 and P15(INK4B)/MTS2 genes in primary human testicular germ cell tumors |
| |
| Authors: | A Heidenreich JP Gaddipati JW Moul S Srivastava |
| |
| Affiliation: | Observatoire Océanologique, Centre National de la recherche Scientifique, Institut National des Sciences de l'Univers et Université Pierre et Marie Curie, Banyuls-sur-Mer, France. lebaron@arago.obs-banyuls.fr |
| |
| Abstract: | Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial enumeration in nonsaline waters and can be applied to both live and dead bacteria. The fluorescence signal/noise ratio was improved when SYTO-9 was used to stain living bacteria in nonsaline waters. Inversely, SYBR-II is more appropriate than SYTO dyes for bacterial enumeration of unfixed and fixed seawater samples. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
