| 酶联免疫吸附法快速检测储存粮食中的污染曲霉 |
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| 引用本文： | 陈福生 罗信昌. 酶联免疫吸附法快速检测储存粮食中的污染曲霉[J]. 中国粮油学报, 1999, 14(1): 51-54 |
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| 作者姓名： | 陈福生 罗信昌 |
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| 作者单位： | 华中农业大学农业微生物重点实验室 |
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| 摘 要： | 以曲霉属特异性抗体为材料，运用酶联免疫吸附法对稻谷、小麦、玉米等几种储存粮食中 胞外多糖含量进行了检测，并和平板计数法的曲霉菌落数进行比较，结果表明，两者间有很好的线性相关性。因此酶联免疫吸附法可用于储存粮食中污染曲霉的快速检测。
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| 关 键 词： | 粮食 曲霉 酶联免疫吸附法 污染 测定 |
Enzyme-Linked Immunosorbent Assay of Infected Genus of Aspergillus in Storage Grains |
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| Chen Fusheng Luo Xinchang Zhou Qi Gan Zhibo. Enzyme-Linked Immunosorbent Assay of Infected Genus of Aspergillus in Storage Grains[J]. Journal of the Chinese Cereals and Oils Association, 1999, 14(1): 51-54 |
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| Authors: | Chen Fusheng Luo Xinchang Zhou Qi Gan Zhibo |
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| Abstract: | The exo-polysaccharide content of Aspergillus genus in stored grains such as rice,wheat and com was detected by Enzyme-linked Immunosorbent Assay(ELISA)with a Aspergillus genus-specific antibody.As a comparison,the colonies of the fungi were detected with Plate Counter Assay.The result revealed that there was a good linear relativity between the exo-polysaccharide content and the number of the colonies.Therefore, the infestation of Aspergillus genus in stored grains could be rapidly detected with ELISA. |
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| Keywords: | grain Aspergillus ELISA |
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