| 液相色谱-串联质谱法快速测定人血浆中丹参酮IIA的含量 |
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| 引用本文： | 宋玉乔,廖杰,黄学亮,管键,赵玉兰.液相色谱-串联质谱法快速测定人血浆中丹参酮IIA的含量[J].现代仪器使用与维修,2011(6):22-24. |
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| 作者姓名： | 宋玉乔 廖杰 黄学亮 管键 赵玉兰 |
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| 作者单位： | [1]解放军总医院医学实验测试中心,北京100853 [2]武警河北省总队医院,石家庄050081 |
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| 摘 要： | 建立测定人血浆中丹参酮IIA含量的LC-MS/MS方法。取正常人血浆200μL，然后加入200ng/mL隐丹参酮溶液（内标）20μL，加入580μL乙腈沉淀，离心10min（1500rpm）后，取上清液吹干溶剂后，用10%乙腈400μL溶解，取5μL进行LC-MS/MS测定。LC条件：采用ASB C18柱（2.1×50mm,5μm）,流动相：乙腈-水梯度洗脱，流速：0.5mL/min.质谱条件：ESI电离源，正离子模式，多反应监测（MRM）方式，
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| 关 键 词： | LC-MS/MS 丹参酮IIA 含量测定 |
Determination of tanshinone IIA in human plasma by LC-MS/MS |
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| Song Yuqiao Liao Jie Huang Xueliang Guan Jian,Zhao Yulan.Determination of tanshinone IIA in human plasma by LC-MS/MS[J].Modern Instruments,2011(6):22-24. |
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| Authors: | Song Yuqiao Liao Jie Huang Xueliang Guan Jian Zhao Yulan |
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| Affiliation: | Song Yuqiao Liao Jie Huang Xueliang Guan Jian Zhao Yulan(1.Medical Experiment and Analysis Center of General Hospital ofPLA, Beijing 100853; 2.The hospital of HeBei Corps of CAPF, Shijiazhuang 050081) |
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| Abstract: | A LC-MS/MS method to fast determine tanshinone IIA in human plasma was established.The internal standard（cryptotanshinone）20μL was added to 200μL plasma,then precipitated with 580μL acetonitrile.After centrifugelling and transfering and evaporating,the residue was dissolved with 400μL 10% acetonitrile and determined by injecting 5μL to LC-MS/MS system.ASB C18 column was used and the mobile phase consisted of acetonitrile water grodient system at the flow rate of 0.5mL/min. |
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| Keywords: | Tanshinone IIA Liquid chromatography-tandem mass spectrometry Determination |
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