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白腐真菌产木聚糖酶的筛选及其发酵条件研究
引用本文:岳晓禹,袁桂英,郭小华,张恒业,刘艳娟,牛天贵.白腐真菌产木聚糖酶的筛选及其发酵条件研究[J].中国酿造,2011(5).
作者姓名:岳晓禹  袁桂英  郭小华  张恒业  刘艳娟  牛天贵
作者单位:1. 郑州牧业工程高等专科学校,质检系,河南,郑州,450011;中国农业大学,食品科学与营养工程学院,北京,100083
2. 河南农业职业学院,河南,郑州,451450
3. 中南民族大学,生命科学学院,湖北,武汉,430074
4. 郑州牧业工程高等专科学校,质检系,河南,郑州,450011
5. 河南商业高等专科学校,河南,郑州,450045
6. 中国农业大学,食品科学与营养工程学院,北京,100083
基金项目:河南省重点技术发展计划,河南省教育厅自然科学研究计划项目,郑州牧专博士科研启动资金项目
摘    要:从白腐真菌中筛选产木聚糖酶菌株,并研究其产酶条件.从保存的白腐真菌中筛选出了一株产木聚糖酶活较高的菌株.通过单因素和正交试验对该菌株的发酵条件进行了研究.结果表明,5%麸皮和玉米芯(1∶1)为最佳碳源,0.5%蛋白胨为最佳氮源,接种量为每50mL发酵液接4块菌丝块,发酵时间为8.5d,pH值为5.4.优化后的发酵条件显著提高了该菌株产木聚糖酶酶活力.

关 键 词:白腐真菌  木聚糖酶  发酵条件  正交试验

Screening and fermentation conditions of xylanase-producing white rot fungi
YUE Xiaoyu,YUAN Guiying,GUO Xiaohua,ZHANG Henye,LIU Yanjuan,NIU Tiangui.Screening and fermentation conditions of xylanase-producing white rot fungi[J].China Brewing,2011(5).
Authors:YUE Xiaoyu  YUAN Guiying  GUO Xiaohua  ZHANG Henye  LIU Yanjuan  NIU Tiangui
Affiliation:YUE Xiaoyu1,2,YUAN Guiying3,GUO Xiaohua4,ZHANG Henye1,LIU Yanjuan5,NIU Tiangui2 (1.Department of Quality Detection and Management,Zhengzhou College of Animal Husbandry Engineering,Zhengzhou 450011,China,2.College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,3.Henan Vocational College of Agriculture,Zhengzhou 451450,4.College of Life Science,South-Central University for Nationalities,Wuhan 430074,5.Henan Business College,Zhengzhou 450045,China)
Abstract:A strain of xylanase producing was screened from white-rot fungi.The fermentation of the xylanase was studied.A xylanase producing strain was isolated from the preservation of white-rot fungi.The fermentation conditions of xylanase by the strain are optimized in the study through the single factor and the orthogonal experiment.So the best conditions are: 2.5% wheat bran + 2.5% corn cob,0.5% peptone,4 pieces per 50mL liquid volume,8.5d,and start pH value 5.4.The optimized fermentation conditions improved sig...
Keywords:white rot fungi  xylanase  fermented conditions  orthogonal experiment  
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