|
|||||
|
|
| 马奶酒中抗单增李斯特氏菌细菌素产生菌的筛选及鉴定 | |
| 引用本文： | 吴敬,芒来,贺银凤.马奶酒中抗单增李斯特氏菌细菌素产生菌的筛选及鉴定[J].中国酿造,2011(4). |
| 作者姓名： | 吴敬 芒来 贺银凤 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018;内蒙古农业大学,食品科学与工程学院,内蒙古,呼和浩特,010018 2. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018 3. 内蒙古农业大学,食品科学与工程学院,内蒙古,呼和浩特,010018 |
| 基金项目： | 内蒙古自然科学基金项目，国家科技部合作项目"走出去"计划项目 |
| 摘 要： | 从内蒙古传统乳制品酸马奶酒中分离出144株乳酸菌,通过双层琼脂平板扩散法筛选出具有明显抑菌作用的菌株.在排除有机酸、H2O2等的干扰后,其中1株乳酸菌的发酵上清液对受试的单核细胞增生李斯特氏菌表现出明显抑制作用;进一步硫酸铵沉淀、透析及浓缩处理后,其抑菌活性显著增强,说明其代谢产物中含有蛋白质类抑菌物质,可能是细菌素.经生理、生化试验和形态学观察,将该菌株鉴定为屎肠球菌(Enterococcus faecium). |
| 关 键 词： | 马奶酒 乳酸菌 细菌素 筛选 鉴定 |
Screening for anti-Listeria bacteriocin-producing lactic acid bacteria from koumiss |
|
| WU Jing,MANG Lai,HE Yinfeng.Screening for anti-Listeria bacteriocin-producing lactic acid bacteria from koumiss[J].China Brewing,2011(4). | |
| Authors: | WU Jing MANG Lai HE Yinfeng |
| Affiliation: | WU Jing1,2,MANG Lai1,HE Yinfeng(1.College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China,2.College of Food Science and Engineering,China) |
| Abstract: | 144 lactic acid bacteria strains isolated from traditional fermented koumiss were screened for bacteriocin production by double agar diffusion method.After eliminating the effects of organic acid and catalase,the cell free supernatant of one strain named MKB63 showed strong anti-Listeria activity and were identified as Enterococcus faecium by physiological-biochemical characterization.After ammonium sulphate precipitation,dialysis and concentration,the activity was enhanced.But the inhibition activity was l... |
| Keywords: | koumiss lactic acid bacteria bacteriocin screening identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |