| 高产胞外葡萄糖-1-磷酸菌株的筛选及鉴定 |
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| 引用本文： | 许向华,史军花,赵国罡,郑鸿雁. 高产胞外葡萄糖-1-磷酸菌株的筛选及鉴定[J]. 中国酿造, 2011, 0(4) |
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| 作者姓名： | 许向华 史军花 赵国罡 郑鸿雁 |
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| 作者单位： | 1. 吉林农业大学,食品科学与工程学院,吉林,长春,130118
2. 吉林大学,农业与生物工程学院,吉林,长春,130022 |
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| 基金项目： | 吉林农业大学青年启动基金项目 |
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| 摘 要： | 采用平板初筛、摇瓶复筛结合TLC和HPLC-MS检测的方法,从有机物丰富的土壤中筛选到一株以麦芽糖为底物直接发酵高产胞外葡萄糖-1-磷酸的菌株;经形态学、生理生化特性及16S rDNA基因序列分析,鉴定该菌株为枯草芽孢杆菌.
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| 关 键 词： | 葡萄糖-1-磷酸 筛选 鉴定 |
Screening and identification of high yield extracellular glucose-1-phosphate strain |
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| XU Xianghua,SHI Junhua,ZHAO Guogang,ZHENG Hongyan. Screening and identification of high yield extracellular glucose-1-phosphate strain[J]. China Brewing, 2011, 0(4) |
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| Authors: | XU Xianghua SHI Junhua ZHAO Guogang ZHENG Hongyan |
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| Affiliation: | XUXianghua1,SHI Junhua1,ZHAO Guogang2,ZHENG Hongyan1(1.College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China,2.School of Biological and Agricultural Engineering,Jilin University,Changchun 130022,China) |
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| Abstract: | A high-yielding extracellular glucose-1-phosphate strain with maltose as substrate was isolated from organic-rich soil by plate screening,shaking flask rescreening and combined with thin-layer chromatography and high performance liquid chromatography-mass spectrum detection.Through morphological,physiological and biochemical properties and 16S rDNA gene sequence analysis,it was identified as Bacillus subtilis. |
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| Keywords: | glucose-1-phosphate(G-1-P) screening identification |
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