| 一种"分瓶"培养与二次接种优化谷氨酸发酵条件的方法 |
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| 引用本文： | 王欣,王燕,高晓娟,杨平平. 一种"分瓶"培养与二次接种优化谷氨酸发酵条件的方法[J]. 中国酿造, 2011, 0(5) |
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| 作者姓名： | 王欣 王燕 高晓娟 杨平平 |
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| 作者单位： | 山东轻工业学院,山东省微生物工程重点实验室,山东,济南,250353 |
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| 摘 要： | 该试验优化了谷氨酸发酵工艺,通过谷氨酸产量的变化寻找细胞由"生长型"转入"生产型"的发酵时间,分时间段与"分瓶"培养并结合二次接种工艺发酵生产谷氨酸,取得较好的发酵结果.最终得到谷氨酸产量113g/L,糖酸转化率58%.
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| 关 键 词： | 溶解氧 谷氨酸 发酵 二次接种 |
Optimization of fermentation conditions of glutamic acid by "sub-bottle" culture and two-step inoculations |
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| WANG Xin,WANG Yan,GAO Xiaojuan,YANG Pingping. Optimization of fermentation conditions of glutamic acid by "sub-bottle" culture and two-step inoculations[J]. China Brewing, 2011, 0(5) |
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| Authors: | WANG Xin WANG Yan GAO Xiaojuan YANG Pingping |
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| Affiliation: | WANG Xin,WANG Yan,GAO Xiaojuan,YANG Pingping(Shandong Provincial Key Lab.of Microbial Engineering,Shandong Institute of Light Industry,Jinan 250353,China) |
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| Abstract: | Fermentation conditions of glutamic acid were optimized in this study.The change of glutamic acid production was the key index for judging the turning time from microbe growth to fermentation.The method combination of the sub-bottle culture with two-step inoculations was used to increase the production of glutamic acid.Finally,the production of glutamic acid was 113g/L and the conversation rate from glucose to glutamic acid reached 58%. |
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| Keywords: | dissolved oxygen glutamic acid fermentation two-step inoculations |
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