| 分子量及其分布的测量方法在生物高分子领域中的应用 |
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| 引用本文： | 王计伟,施庆珊,欧阳友生,胡文锋,陈仪本.分子量及其分布的测量方法在生物高分子领域中的应用[J].广东化工,2011,38(4):18-20. |
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| 作者姓名： | 王计伟 施庆珊 欧阳友生 胡文锋 陈仪本 |
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| 作者单位： | 王计伟,Wang Jiwei(广东省微生物研究所所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东,广州,510070;华南农业大学,食品学院,广东,广州,510640);施庆珊,欧阳友生,陈仪本,Shi Qingshan,Ouyang Yousheng,Chen Yiben(广东省微生物研究所所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广东,广州,510070);胡文锋,Hu Wenfeng(华南农业大学,食品学院,广东,广州,510640) |
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| 摘 要： | 分子量是生物高分子性质的一项重要参数,决定了生物高分子的许多理化特性和生物效应.准确测定生物高分子的分子量有利于了解其性质,是其有效应用的前提.文章主要简述了近年来常用的测定生物高分子分子量的方法,如黏度测量法、凝胶渗透色谱法、基质辅助激光解暖-飞行时间质谱法、电喷雾电离质谱法,并对其进行分析和比较,为微生物合成生物高...
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| 关 键 词： | 生物高分子 分子量 分子量分布 测定方法 |
Methods for Determining Molecular Weight and Molecularweight Distributions and Their Applications in the Field of Bio-polymers |
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| Wang Jiwei,Shi Qingshan,Ouyang Yousheng,Hu Wenfeng,Chen Yiben.Methods for Determining Molecular Weight and Molecularweight Distributions and Their Applications in the Field of Bio-polymers[J].Guangdong Chemical Industry,2011,38(4):18-20. |
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| Authors: | Wang Jiwei Shi Qingshan Ouyang Yousheng Hu Wenfeng Chen Yiben |
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| Affiliation: | 1(1.Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Provincial Key Laboratory of Applied Microbiology,Guangdong Institute of Microbiology,Guangzhou 510070;2.College of Food Science,South China Agricultural University,Guangzhou 510640,China) |
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| Abstract: | Molecular weight is an important parameters of nature for biopolymers,which determines the physical and chemical properties of biopolymers and much biological effects.Determinating the molecular weight of biopolymers accurately is conducive to know the nature,that is a prerequisite for its effective application.The paper outlined common in recent years,the methods for determinating molecular weight of biopolymers,such as viscosity measurements,gel permeation chromatography,matrix-assisted laser desorption ionization-time of flight mass spectrometry,electrospray ionization mass spectrometry,and also analyzed and compared these methods,in order to provide a basis for determinating bio-molecular weight and molecular weight distribution in the field of the microbial synthetic polymers. |
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| Keywords: | biopolymers molecular weight molecular weight distribution determination methods |
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