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Engineering global regulators for enhanced tolerance to multiple inhibitors by CRISPR-enabled trackable genome engineering |
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| Authors: | Xin Song Yangyang Zheng Shuting Li Alaksh Choudhury Xunli Liu Tao Chen Ryan T. Gill Zhiwen Wang |
| Affiliation: | 1. Frontier Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering (Ministry of Education), Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China College of Forestry, Shandong Agricultural University, Taian, Shandong Province, China Contribution: Data curation (lead), Formal analysis (lead), Investigation (equal), Methodology (equal), Project administration (equal), Resources (equal), Software (equal), Validation (equal), Writing - original draft (equal);2. Frontier Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering (Ministry of Education), Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China Contribution: Data curation (equal), Formal analysis (equal), Investigation (equal), Project administration (equal), Writing - original draft (lead), Writing - review & editing (lead);3. Frontier Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering (Ministry of Education), Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China Contribution: Data curation (equal), Formal analysis (equal), Project administration (equal), Resources (equal), Software (equal), Writing - original draft (equal);4. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, Colorado, USA Contribution: Investigation (supporting), Software (lead), Writing - original draft (equal);5. College of Forestry, Shandong Agricultural University, Taian, Shandong Province, China Contribution: Conceptualization (equal), Project administration (equal), Supervision (equal);6. Frontier Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering (Ministry of Education), Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China Contribution: Conceptualization (equal), Funding acquisition (equal), Resources (equal), Supervision (equal);7. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, Colorado, USA Contribution: Methodology (equal), Visualization (equal);8. Frontier Science Center for Synthetic Biology (Ministry of Education), Key Laboratory of Systems Bioengineering (Ministry of Education), Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China |
| Abstract: | |
| Keywords: | CRISPR-enabled trackable genome engineering (CREATE) Escherichia coli global regulator regulation tolerance |