固定酶法催化胆固醇合成胆固醇油酸酯的研究

探讨了固定化Camdoda antarctica脂肪酶催化胆固醇与油酸合成胆固醇油酸酯,通过脂肪酶催化酯化反应的工艺路线进行研究,系统考察了反应溶剂、醇油比、催化剂用量、反应温度等对酯化反应的影响.结果表明,以环己烷为溶剂,30℃,物质的量比(胆固醇:油酸)为1:1,2%(质量分数)脂肪酶,反应时间48 h,反应的最高酯化率可达到88%以上,产品经HPLC和LC-MS分析的纯度可达98%以上,固定化Candida ant-arctica脂肪酶具有较高的催化的催化稳定性,其半衰期至少达100 d.

Synthesis of Cholesterol Oleate from Cholesterol and Oleic Acid by Using Immobilized Candida antarctica Lipase

The esterification process of cholesterol and oil acid to synthesize cholesterol oleate catalyzed by im-mobilized C.Antarctica lipase was investigated.The effects of different organic solution,enzyme dose,reaction tem-perature,and molar ration of substrate on the esterification were studied.Results:Using cyclohexane as solvent,un-der conditions of 30℃,enzyme dose 2%(w/w) and reaction time 36 h,the esterification rate is up to 88%,and the product purity evaluated by HPLC and LS-MS is up to 98%.The immobilized C.Antarctica lipase has half-life of at least 100 d,exhibiting good operational stability in the reaction system.