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Solution-stirring method improves crystal quality of human triosephosphate isomerase |
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| Authors: | Adachi Hiroaki Niino Ai Kinoshita Takayoshi Warizaya Masaichi Maruki Riyo Takano Kazufumi Matsumura Hiroyoshi Inoue Tsuyoshi Murakami Satoshi Mori Yusuke Sasaki Takatomo |
| Affiliation: | SOSHO Project (Crystal Design Project), Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. |
| Abstract: | We examined the effect of a solution-stirring method on human triosephosphate isomerase crystallization. The crystals diffracted to more than 1.4 A resolution, whereas those obtained by the normal vapour-diffusion technique diffracted to 2.8 A. These results clearly show that the solution-stirring method is valuable and useful for protein crystallization because of its effectiveness and simplicity. |
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