Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires |
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Authors: | Syed M. Usman AliAuthor Vitae N.H. AlviAuthor VitaeZafar IbupotoAuthor Vitae Omer NurAuthor VitaeMagnus WillanderAuthor Vitae Bengt DanielssonAuthor Vitae |
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Affiliation: | a Physical Electronics and Nanotechnology Division, Department of Science and Technology, Campus Norrköping, Linköping University, SE-60174 Norrköping, Sweden b Acromed Invest AB, Magistratsv. 10, SE-226 43 Lund, Sweden |
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Abstract: | In this study, a potentiometric uric acid biosensor was fabricated by immobilization of uricase onto zinc oxide (ZnO) nanowires. Zinc oxide nanowires with 80-150 nm in diameter and 900 nm to 1.5 μm in lengths were grown on the surface of a gold coated flexible plastic substrate. Uricase was electrostatically immobilized on the surface of well aligned ZnO nanowires resulting in a sensitive, selective, stable and reproducible uric acid biosensor. The potentiometric response of the ZnO sensor vs Ag/AgCl reference electrode was found to be linear over a relatively wide logarithmic concentration range (1-650 μM) suitable for human blood serum. By applying a Nafion® membrane on the sensor the linear range could be extended to 1-1000 μM at the expense of an increased response time from 6.25 s to less than 9 s. On the other hand the membrane increased the sensor durability considerably. The sensor response was unaffected by normal concentrations of common interferents such as ascorbic acid, glucose, and urea. |
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Keywords: | ZnO nanowires Potentiometric nanosensor Uricase Uric acid Nafion® Membrane Electrochemical nanodevices |
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