SERS driven cross-platform based multiplex pathogen detection |
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Authors: | Sandeep P Ravindranath Author VitaeYuling Wang Author Vitae Joseph IrudayarajAuthor Vitae |
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Affiliation: | Department of Agricultural and Biological Engineering, Bindley Bioscience Center, Purdue University, 225 S. University Street, West Lafayette, IN 47907, United States |
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Abstract: | We demonstrate a cross-platform approach to simultaneously detect three different pathogens using Raman and UV-vis absorption spectroscopy. Gold (Au), silver (Ag), and Ag-Au core-shell nanoparticles were functionalized with anti-Salmonella typhimurium aptamers, anti-Staphylococcus aureus and anti-Escherichia coli O157:H7 antibodies respectively and labeled with unique Raman reporter molecules. A microfiltration step was used to consolidate a highly selective and specific detection platform, with total detection time under 45 min for both species (E. coli O157:H7 vs. S. typhimurium) and strain (E. coli O157:H7 vs. E. coli K12) level sensing at a limit of a detection ranging between 102 and 103 CFU/ml. This simple yet robust multiplex detection platform has the potential to be developed into a rapid and portable pathogen sensor for ultrasensitive detection in liquid samples. |
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Keywords: | Pathogens SERS Multiplex detection Aptamers Nanotechnology Cross-platform detection |
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