虫茶醇提取物对超氧阴离子和羟基自由基的清除作用

目的:本文对老鹰茶虫茶醇提取物对超氧阴离子和羟基自由基的清除作用进行了研究。方法:以老鹰茶虫茶为原料,以不同浓度的乙醇溶液粗提物,其醇制浸膏采用邻苯三酚法测定O2—、邻二氮菲法测OH体系。结果:老鹰茶虫茶40%乙醇提取物得率为最高。超氧阴离子的清除趋势、活性不一。清除OH的能力依次为:无水乙醇提取物20%乙醇提取物80%乙醇提取物60%乙醇提取物40%乙醇提取物。结论:无论是何种浓度乙醇提取物都表现出了良好的清除超氧阴离子和羟基自由基的能力。各种浓度相比之下,20%乙醇提取物对超氧阴离子清除能力较好,无水乙醇提取物对羟基自由基的清除能力较好。

Scavenging Effects of Sandy-tea Ethanol Extract on Super Oxide Anion and Hydroxyl Radical

Objective： This paper studies on the scavenging effects of Hawk-tea-Sandy-tea ethanol extract on Super oxide Anion and Hydroxyl Radical. Methods： For the materials with Hawk-tea-Sandy-tea, then using pyrogallol method to measure super oxide anion radical （O2^-）, orthophenanthroline reaction method to measure hydroxyl radical（.OH）by the different solvent concentration extracts. Results： The rate of 40%Ethanol extract is the highest. The clear trend and active of Super oxide anion is different. The scavenge ability of different solvent concentration extract with ·OH is Ethanol extract〉20%Ethanol extract〉80%Ethanol extract〉60%Ethanol extract〉40%Ethanol extract. Conclusion： Either Super oxide anion or Hydroxyl radicals will be scavenged very well by any concentration ethanol extract. Compared with various concentrations, 20%Ethanol extract is better on scavenging Super oxide anion and Ethanol extract is better on scavenging Hydroxyl radical.