Determination of molecular species of oil triacylglycerols by reversed-phase and chiral-phase high-performance liquid chromatography |
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Authors: | G Semporé J Bézard |
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Affiliation: | (1) Unite de Nutrition Cellulaire et Métabolique, Université de Bourgogne, BP 138 21004, Dijon, Cedex, France |
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Abstract: | A method is described for the determination of molecular species of oil triacylglycerols. The method is based on the analytical
separation of the enantiomericsn-1,2-andsn-2,3-diacylglycerols, derived from triacylglycerols, by high-performance liquid-chromatography (HPLC) on a chiral column containing
N-(R)-1-(α-naphthyl)ethylaminocarbonyl-(S)-valinecarbonyl-(S)-valine as stationary phase. Model triacyl-glycerol molecules
comprising three known fatty acids were isolated from peanut oil and cottonseed oil by a combination of argentation-TLC and
reversed-phase HPLC and submitted to partial chemical deacylation. The derivedsn-1,2(2,3)-diacylglycerols were analyzed and fractionated as 3,5-dinitrophenyl urethane derivatives by reversed-phase HPLC
according to chainlength and unsaturation. From thesn-1,2(2,3)-diacylglycerol composition and the diacylglycerolsn-1,2-andsn-2,3-enantiomer composition, the individual molecular species of four peanut oil triacylglycerols and one cottonseed oil triacylglycerol
were identified and quantitated. The method can be applied to triacylglycerols of any other oil or fat. |
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Keywords: | Chiral-phase HPLC diacylglycerols enantiomers molecular species reversed-phase HPLC triacylglycerols |
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