葛根超微粉、葛粉中葛根素、大豆甙和大豆甙元的分析研究

采用高效液相色谱法测定功能食品葛根超微粉、葛粉中的葛根素、大豆甙和大豆甙元,为葛根保健品的质量控制提供方法保证。试验利用70%乙醇溶液超声提取样品,流动相为甲醇与水二元体系。结果显示,葛根超微粉中葛根素、大豆甙和大豆甙元的含量远远高于葛粉。加标回收率为90.1%~100.5%,该方法操作简便,准确可靠。

Study on puerarin,daidzin and daidzein in radix pueraria and pueraria powder

The content of puerarin,daidzin and daidzein in function foods radix pueraria and pueraria powder was determined by HPLC.It could provide a method of quality control for pueraria health products.The sample was extracted by ultrasound with 70% ethanol for 30 min.The mobile phase consisted of methanol and water.The contents of puerarin,daidzin and daidzein in the radix pueraria are far higher than those in the pueraria powder.The results showed that the recoveries were in the range of 90.1%～100.5%.The method is simple and accurate.