| 辐射交联聚己内酯的酶降解动力学研究 |
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| 引用本文： | 张龙彬,朱光明,许硕贵,王金花,秦瑞丰. 辐射交联聚己内酯的酶降解动力学研究[J]. 辐射研究与辐射工艺学报, 2006, 24(5): 275-278 |
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| 作者姓名： | 张龙彬 朱光明 许硕贵 王金花 秦瑞丰 |
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| 作者单位： | 西北工业大学应用化学系,西安,710072;第二炮兵工程学院,西安,710025;西北工业大学应用化学系,西安,710072;第二军医大学长海医院,上海,200433 |
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| 摘 要： | 研究了聚己内酯（Polycaprolactone，PCL）和辐射交联PCL的脂肪酶降解行为及酶促反应动力学，得到了PCL降解速度与时间和底物浓度的关系及Michaelis-Menten常数。实验发现，降解产物浓度随着降解时间和底物浓度增加而增加。PCL粉末和交联PCL颗粒的降解动力学较好地符合米氏方程，其动力学参数Km分别为1．14、71．95mg／mL，最大降解速度Vmax分别为0．39、0．96mL／h。
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| 关 键 词： | 聚己内酯 辐射交联 酶降解 动力学 |
| 收稿时间： | 2006-01-10 |
| 修稿时间： | 2006-05-15 |
Kinetic study for enzymatic degradation of PCL |
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| ZHANG Longbin,ZHU Guangming,XU Shuogui,WANG Jinhua,QIN Ruifeng. Kinetic study for enzymatic degradation of PCL[J]. Journal of Radiation Research and Radiation Processing, 2006, 24(5): 275-278 |
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| Authors: | ZHANG Longbin ZHU Guangming XU Shuogui WANG Jinhua QIN Ruifeng |
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| Abstract: | Kinetics of enzymatic degradation behavior of cross-linked PCL was studied. It is well known that the enzymatic hydrolysis of water-insoluble PCL by depolymerases produces water-soluble products. The result showed that the concentration of product in the solution increased with the time and substrates concentration. The degradation kinetics followed Mechaelis-Menten equation. The degradation parameter of the PCL powder was 1.14mg/mL for Km and 0.39mL/h for Vmax, respectively. And the degradation parameter of cross-linked PCL particulate was 71.95mg/mL for Krn and 0.96mL/h for Vmax, respectively. |
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| Keywords: | Polycaprolactone Enzymatic degradation Kinetics Titrimetry |
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