| 淀粉废水发酵产细菌纤维素发酵条件的优化 |
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| 引用本文： | 淀粉废水发酵产细菌纤维素发酵条件的优化[J]. 食品工业科技, 2012, (20): 184-187. DOI: 10.13386/j.issn1002-0306.2012.20.061 |
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| 作者姓名： | 徐伟 张妍 傅徐阳 |
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| 作者单位： | 1. 哈尔滨商业大学食品工程学院,黑龙江哈尔滨,150076
2. 国家乳制品质量监督检验中心,黑龙江哈尔滨,150086 |
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| 摘 要： | 以玉米淀粉废水添加葡萄糖20g/L,玉米浆40g/L,乙醇150mL/L为发酵基质,采用单因素和正交实验设计对葡糖醋杆菌(Gluconacetobacter xylinus)发酵产细菌纤维素条件进行优化。结果表明,最佳发酵条件为:装液量80mL/250mL,pH4.0,接种量9%(V/V),温度28℃;在此条件下得到细菌纤维素产量为4.41g/L。采用傅立叶转换红外光谱FTIR验证产物为细菌纤维素,并由SEM扫描电镜观察纤维素膜表面形貌。
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| 关 键 词： | 玉米淀粉废水 细菌纤维素 条件优化 FTIR SEM |
| 收稿时间： | 2012-07-26 |
Optimization of culture conditions of producing bacterial cellulose utilizing starch wastewater |
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| Optimization of culture conditions of producing bacterial cellulose utilizing starch wastewater[J]. Science and Technology of Food Industry, 2012, (20): 184-187. DOI: 10.13386/j.issn1002-0306.2012.20.061 |
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| Authors: | XU Wei ZHANG Yan FU Xu-yang |
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| Affiliation: | 1.Food Engineering Institute,Harbin Commercial University,Harbin 150076,China; 2.National Dairy Testing Center,Harbin 150086,China) |
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| Abstract: | The culture conditions of Gluconacetobacter xylinus producing bacterial cellulose utilizing corn starch wastewater (adding glucose 20g/L, corn steep liquor 40g/L, ethanol 150mL/L) were investigated through single- factor and orthogonal tests. The suitable culture conditions were as follows:liquid level was 80mL in 250mL triangle bottle, pH4.0, inoculation volume was 9% (V/V) , culture temperature was 28℃ , the the yield of bacterial cellulose reached the peak (4.41g/L) at this time. The bacterial cellulose was verified by FTIR, SEM was used to observe the surface pattern of bacterial cellulose membrane. |
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| Keywords: | corn starch wastewater bacterial cellulose optimization of conditions FTIR SEM |
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