响应面法优化蜂胶黄酮提取工艺及其抗氧化活性研究

采用响应面法对蜂胶黄酮提取工艺进行优化。以蜂胶为原料,基于单因素实验,以乙醇体积分数、液料比、提取时间和提取温度为相应因素、蜂胶黄酮提取率为响应值,采用四因素三水平的响应面分析法,确定最佳提取工艺条件为:液料比14:1（mL·g-1）、提取温度72.6℃、提取时间2.5h、乙醇浓度80%（v/v）,在此条件下理论提取率为33.9324%,与实测值基本相符,说明优化工艺可行。以VC为对照物,经DPPH法和铁氰化钾法验证蜂胶黄酮的抗氧化活性,发现蜂胶黄酮具有较强的清除DPPH自由基能力和还原能力,可作为优良的天然抗氧化剂资源。 

Optimization of extraction process of propolis flavonoids by response surface methodology and its antioxidant activity research

Propolis flavonoids were extracted by ethanol, and the extraction process was optimized by response surface analysis.On the basis of one-factor-at-a-time experiment, optimal extraction conditions for maximizing popolis flavonoids were determined by using a 4-variable, 3-level Box-Behnken experimental design combined with response surface analysis as follows:the liquid-material ratio was 14:1 (mL·g-1) , the extraction temperature was 72.6℃, the extraction time was 2.5h, the ethanol concentration was 80% (v/v) , resulting in an propolis flavonoids yield of 33.82%, which was basic agreement with the predicted value of 33.9324%.Thus, the optimal process was reliable.The antioxidant activity in vitro condition of propolis flavonoids were evaluated by DPPH free radical scavenging assay and potassium ferricyanide reduction assay using V C as the control.Propolis flavones were found to have powerful DPPH free radical scavenging capacity and reducing power.Thus, propolis flavonoids could be developed as excellent natural antioxidant resources.