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卷须链霉菌木聚糖酶水解玉米芯汽爆液及酵母发酵精制低聚木糖
引用本文:张艳艳,薛文通,江正强,李里特,丁长河.卷须链霉菌木聚糖酶水解玉米芯汽爆液及酵母发酵精制低聚木糖[J].食品与发酵工业,2004,30(4):12-16.
作者姓名:张艳艳  薛文通  江正强  李里特  丁长河
作者单位:中国农业大学食品科学与营养工程学院,北京,100083
基金项目:国家“十五”科技攻关计划项目 (No.2 0 0 1BA70 8B0 4 - 0 6 )
摘    要:研究了卷须链霉菌 (Streptomycescirratus)D 1 0木聚糖酶水解玉米芯汽爆液的酶用量和水解时间 ,结果得到 80U/ 1 0 0mL酶用量和水解 6h是合适的加酶量和水解时间 ;筛选出 1株能够精制低聚木糖的Candiasp .酵母 ,该酵母能消耗木糖、阿拉伯糖而不消耗木二糖 ;利用此酵母精制酶解液 ,在接菌量为 1 0 %,48h时酶解液中木糖、阿拉伯糖完全被消耗 ,木二糖以上的低聚木糖含量变化不明显 ,因此利用该酵母能达到精制低聚木糖的效果

关 键 词:卷须链霉菌  木聚糖酶  玉米芯汽爆液  酵母  低聚木糖
修稿时间:2003年10月8日

The Purification of the Hydrolyate from Hydrolyzing Steam-exploded Corncob by Streptomyces cirratus D-10
Zhang Yanyan Xue Wentong Jiang ZhengqiangLi Lite,Ding Changhe.The Purification of the Hydrolyate from Hydrolyzing Steam-exploded Corncob by Streptomyces cirratus D-10[J].Food and Fermentation Industries,2004,30(4):12-16.
Authors:Zhang Yanyan Xue Wentong Jiang ZhengqiangLi Lite  Ding Changhe
Abstract:The conditions of Streptomyces cirratus D 10 xylanase hydrolyzing steam exploded corncob were studied.It shows that the proper amount of xylanase and the appropriate hydrolyzed time were 80U/mL and 6h,respectively. A strain of Candia sp. was selected in this study. This strain can effectively and selectively metabolize all xylose and arabinose but not xylobiose,so it could be used to purify the hydrolyzed steam exploded corncob. Xylose and arabinose in the hydrolysate were fully consumed with 10% inoculum at 48h,while the mount of the xylooligosaccharides unchanged.
Keywords:Streptomyces  cirratus  xylanses  steam  exploded  corncob  yeast  xylooligosaccharides
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