| 皱纹盘鲍内脏脂质对人肝癌细胞HepG2脂质调节作用研究 |
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| 引用本文： | 陈贝,张伯超,吴毓敏,乔琨,曲海东,许晏,潘南,刘智禹.皱纹盘鲍内脏脂质对人肝癌细胞HepG2脂质调节作用研究[J].现代食品科技,2019,35(4):22-29. |
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| 作者姓名： | 陈贝 张伯超 吴毓敏 乔琨 曲海东 许晏 潘南 刘智禹 |
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| 作者单位： | 福建省水产研究所,国家海水鱼类加工技术研发分中心(厦门),福建省海洋生物增养殖与高值化利用重点实验室,福建省海洋生物资源开发利用协同创新中心,福建厦门361013;闽南师范大学生物科学与技术学院,福建漳州,363000;厦门大学环境与生态学院,福建厦门,361013 |
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| 基金项目： | 海洋公益性行业科研专项（201405016） |
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| 摘 要： | 本研究探讨了皱纹盘鲍(Haliotis discus hannai)内脏脂质(Haliotis discus hannai visceral lipid,HDHL)对人肝癌细胞HepG2的脂质代谢的影响,对HDHL脂肪酸成分、HepG2细胞活性、细胞内胆固醇和甘油三酯含量以及脂肪酸代谢相关m RNA基因的表达情况进行了研究。研究发现HDHL中不饱和脂肪酸占总脂肪酸含量59.50%,其中多不饱和脂肪酸占29.71%。HDHL与HepG2细胞共孵培养24 h及48 h后,浓度为0～240μg/m L的HDHL对HepG2细胞无毒性影响。30～240μg/m L HDHL可显著降低HepG2总胆固醇含量,30～120μg/m LHDHL共孵HepG2细胞后,细胞内甘油三酯含量显著降低。q PCR结果显示HDHL可显著降低HepG2细胞脂肪酸合成相关基因SREBP1c、ACC1、FAS和脂肪酸转运吸收相关基因CD36 m RNA水平的表达,提高线粒体脂肪酸氧化基因CPT1的表达。因此,HDHL可能通过抑制脂肪酸合成和脂肪酸转运,增强线粒体中脂肪酸氧化等途径有效调节细胞脂质代谢,该研究为脂质调节功能食品的开发提供理论依据。
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| 关 键 词： | 人肝癌细胞 皱纹盘鲍 内脏脂质 脂质代谢 |
| 收稿时间： | 2018/11/8 0:00:00 |
Lipid-Modulating Effects of Visceral Lipids from Haliotis discus hannai on HepG2 Cells |
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| CHEN Bei,ZHANG Bo-chao,WU Yu-min,QIAO Kun,QU Hai-dong,XU min,PAN Nan and LIU Zhi-yu.Lipid-Modulating Effects of Visceral Lipids from Haliotis discus hannai on HepG2 Cells[J].Modern Food Science & Technology,2019,35(4):22-29. |
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| Authors: | CHEN Bei ZHANG Bo-chao WU Yu-min QIAO Kun QU Hai-dong XU min PAN Nan and LIU Zhi-yu |
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| Affiliation: | (1.National Research and Development Center for Marine Fish Processing (Xiamen), Key Laboratory of Cultivation and Highvalue Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361013, China),(1.National Research and Development Center for Marine Fish Processing (Xiamen), Key Laboratory of Cultivation and Highvalue Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361013, China),(2. School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou363000, China),(1.National Research and Development Center for Marine Fish Processing (Xiamen), Key Laboratory of Cultivation and Highvalue Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361013, China),(3.College of the Environment and Ecology, Xiamen University, Xiamen 361013, China),(1.National Research and Development Center for Marine Fish Processing (Xiamen), Key Laboratory of Cultivation and Highvalue Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361013, China),(1.National Research and Development Center for Marine Fish Processing (Xiamen), Key Laboratory of Cultivation and Highvalue Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361013, China) and (1.National Research and Development Center for Marine Fish Processing (Xiamen), Key Laboratory of Cultivation and Highvalue Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361013, China) |
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| Abstract: | |
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| Keywords: | human hepatoma cell HepG2 Haliotis discus hannai visceral lipids lipid metabolism |
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