A Partitioned Slab Apparatus for One-Dimensional Polyacrylamide Gel Electrophoresis in Multiphasic Buffer Systems under a Wide Range of Conditions

Abstract

An apparatus for one-dimensional polyacrylamide gel electrophoresis in multiphasic buffer systems of multiple samples on a gel slab has been constructed. This apparatus is made of Pyrex, providing satisfactory wall adherence of polyacrylamide at any pH, 0 and 25°C. The apparatus provides permanent sample partitions and can, therefore, be used with mechanically labile, stacking gels. Temperature control for polymerization and electrophoresis is provided. Procedures and applications are described for use of the apparatus with uniform gel concentration or with pore gradient gels. A gradientmaker for the preparation of polyacrylamide pore gradient gels is described.