The Instrument Spreading Correction in GPC. II. The General Shape Function Using the Fourier Transform Method with a Nonlinear Calibration Curve

Abstract

The instrument spreading function suggested in Part I of this series is investigated for use with the Fourier transform method for generating corrected elution volume chromatograms. The instrument spreading parameters are obtained using linear theory on narrow molecular weight distribution standards, as indicated in Part I. The corrected chromatogram is then combined with a nonlinear molecular weight calibration curve which was fit with a function suggested by Yau and Malone to generate true values of the number- and weight-average molecular weights.

The instrument spreading function is shown to qualitatively and quantitatively describe the dispersion, skewing, and flattening effects ordinarily found in GPC chromatograms due to imperfect resolution by the GPC columns. The Yau-Malone function is shown to be a very useful function for fitting nonlinear molecular weight vs elution volume calibration data. Although the Fourier transform method is shown to work well with analytically generated data, it is shown that a number of numerical problems must be overcome before it can quantitatively produce corrected elution volume chromatograms. Some of these numerical problems are discussed.