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Protein expression by bone mesenchymal stem cells cultivated in PLLA scaffolds with different pore geometry
Abstract:Abstract

The effects of poly(L,L-lactide) (PLLA) scaffold with axial and isotropic structure were investigated on functional activity of rabbit bone mesenchymal stem cells (BMSCs). PLLA scaffolds were processed by freeze-dry technique at different temperatures of the scaffold frost – ?196?°C, ?25?°C and 0?°C. Scaffolds with different pore sizes were obtained by adding 5 or 10% of water phase. Scaffolds were modified by collagen type I solution. The pore sizes of polymer scaffolds were ranging from 5 to 150?µm. More protein secretion was observed in the surface-modified scaffolds than in the unmodified after 2 weeks of cultivation in vitro.
Keywords:Bone marrow mesenchymal stromal cells  extracellular proteins  poly(L,L-lactide) scaffolds  pore geometry  porosity
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