液相色谱法测定生鲜肉中苯甲酸

建立利用液相色谱法对生鲜肉中苯甲酸含量进行测定的方法。该方法中,样品经过提取,加入沉淀剂后,利用低温高速离心法除去样品中的蛋白质、脂肪,效果非常显著,以Waters Symmetry C18色谱柱,以甲醇和乙酸铵水溶液（5：95,V：V）为流动相进行分离,流速1.0 mL/min,紫外检测器于230 nm波长下进行检测。通过检测,在苯甲酸保留时间处无干扰峰,生鲜肉中苯甲酸在0.5～200μg/mL内有良好的线性关系,峰面积与苯甲酸浓度的回归方程为Y=7.51×104X-9.31×103,相关系数为0.99998,RSD为1.2%,得到回收率为90.2-102.9%。

Liquid Chromatography Determination of Benzoic Acid in Fresh Meat

A method is reported for determining the content of Benzoic acid in meat by HPL.After extraction and add-ing precipitation agent,the sample was processed by low-temperature and high-speed centrifugation to remove the protein and fat,and the result was obvious.A Waters Symmetry C18 column was used with Methanol and aqueous ammonium ac-etate(5:95,V:V) as mobile phase.The flow rate was 1.0 mL/min.An UV detector was used and the detection wavelength was 230nm.By detecting,there not is interference peak at the r...