| 异羟肟酸比色法测定D-核糖发酵液中葡萄糖酸的含量 |
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| 引用本文： | 张宗玉,李志敏,叶勤. 异羟肟酸比色法测定D-核糖发酵液中葡萄糖酸的含量[J]. 食品与发酵工业, 2005, 31(8): 81-83 |
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| 作者姓名： | 张宗玉 李志敏 叶勤 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 摘 要： | 对异羟肟酸比色法测定葡萄糖酸含量的方法进行了改进。通过考察不同葡萄糖浓度对异羟肟酸比色法测定葡萄糖酸的影响,得出葡萄糖浓度对葡萄糖酸测定影响的干扰方程,据此计算得到的葡萄糖酸量可以排除葡萄糖的影响,使得检测结果更为准确。方法适用于D-核糖发酵液中葡萄糖酸的测定。
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| 关 键 词： | 葡萄糖酸 异羟肟酸比色法 葡萄糖 |
| 收稿时间： | 2005-01-11 |
| 修稿时间： | 2005-06-13 |
Determination of Gluconate Concentration in Broth of D-ribose Fermentation Using the Hydroxamate Method |
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| Zhang Zongyu,Li Zhimin,Ye Qin. Determination of Gluconate Concentration in Broth of D-ribose Fermentation Using the Hydroxamate Method[J]. Food and Fermentation Industries, 2005, 31(8): 81-83 |
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| Authors: | Zhang Zongyu Li Zhimin Ye Qin |
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| Abstract: | Glucose interferes with gluconate measurement using the hydroxamate colorimetric method. This study focused on the effect of glucose on the measurement of gluconate. Through measuring a series of solutions containing different amount of glucose and gluconate, a formula for calculating gluconate concentration was obtained, with which the interference caused by glucose could be eliminated. This method was applied to determination of gluconate concentration profile during D- ribose fermentation. |
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| Keywords: | gluconate hydroxamate colorimetric method glucose |
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