| 高效液相色谱法测定蛋卷中的苋菜红和日落黄 |
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| 引用本文： | 陈晓珍,郭新东,罗海英,黄金凤,吴玉銮,王永华. 高效液相色谱法测定蛋卷中的苋菜红和日落黄[J]. 现代食品科技, 2007, 23(6): 78-80 |
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| 作者姓名： | 陈晓珍 郭新东 罗海英 黄金凤 吴玉銮 王永华 |
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| 作者单位： | 广州市产品质量监督检验所,国家加工食品质量监督检验中心(广州),广州,510110;华南理工大学轻工与食品学院,广东,广州,510641 |
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| 摘 要： | 建立了测定蛋卷中合成色素日落黄和苋菜红的高效液相色谱分析方法.蛋卷中的合成色素经聚酰胺吸附提取,制成水溶液,过滤后用高效液相色谱仪测定.采用ODS柱分离,甲醇和乙酸铵溶液(0.02 mol/L)为流动相,测定蛋卷中苋菜红和日落黄,结果的相对标准偏差为1.5 %～5.9 %,实际蛋卷样品的回收率为71.5 %～81.4 %,检出限均为0.5 mg/kg.
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| 关 键 词： | 高效液相色谱法 蛋卷 合成色素 |
| 文章编号： | 1673-9078（2007）06-0078-04 |
| 收稿时间： | 2007-04-19 |
| 修稿时间： | 2007-04-19 |
Determination of Artificial Synthetic Pigments (Sunset Yellow and Amaranth) in Egg Roll by HPLC |
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| CHEN Xiao-zhen,GUO Xin-dong,LUO Hai-ying,HUANG Jin-feng,WU Yu-luan,WANG Yong-hua. Determination of Artificial Synthetic Pigments (Sunset Yellow and Amaranth) in Egg Roll by HPLC[J]. Modern Food Science & Technology, 2007, 23(6): 78-80 |
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| Authors: | CHEN Xiao-zhen GUO Xin-dong LUO Hai-ying HUANG Jin-feng WU Yu-luan WANG Yong-hua |
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| Affiliation: | 1 .National Centre for Quality Supervision and Testing of Processed Food (Guangzhou |
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| Abstract: | A method for determination of two artificial synthetic pigments in egg roll (Sunset Yellow and Amaranth) by HPLC was established. the pigments in egg roll After purified was extracted by polyamide, dissolved in water, filtrated and then analyzed by HPLC with a ODS C18 column and the elution of methanol and ammonium acetate (0.02 mol/L). The detection limits, recovery and precision were 0.5 mg/kg, 71.5%~81.4%, and 1.5%~5.9%, respectively. |
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| Keywords: | HPLC egg roll artificial synthetic pigment sunset yellow amaranth |
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