共振拉曼光谱法检测人体皮肤中类胡萝卜素的研究

类胡萝卜素作为一种强抗氧化剂在人体的抗氧化防御系统中起重要作用。它在人体中的含量可以用来表征人体的健康状况。为了检测人体中的类胡萝卜素，对用共振拉曼光谱法检测人体皮肤中类胡萝卜素进行了实验研究。经过反复实验获得了最佳实验参数。如选择测量部位拇指内侧，入射波长473nm，入射光强度20mW，曝光时间5s。同时通过甘油匹配的方法增加了探测到的拉曼信号的强度。利用拟合差分算法有效去除了荧光背景噪声，获得了明显的人体皮肤中类胡萝卜素的拉曼光谱。

Study of resonance raman detection of carotenoid in human

Carotenoid molecules are powerful antioxidants that play important roles throughout the human body. The level of carotenoid in human body can be used to reflect the human’s health condition. The experiment studies have carried out on this method for detecting the carotenoid in human. After tests repeatedly, we have carried on the suitable choice to the experimental parameter. The best location to perform the measurement is thrum, laser’s wavelength is 473nm, intensity of laser is 20mW and exposure time is 5s. At the same time we have increased the intensity of raman signal by daubing glycerin on thrum. At last we obtained obvious resonance Raman spectrum of carotenoid in human skin after the fluorescence background has been fitted and subtracted from the original spectra.