一种化妆水微生物检验方法研究

建立了一种化妆水中微生物的检验方法。以含质量分数为0.1%的吐温80的生理盐水为冲洗液,并用薄膜过滤法对化妆水中的防腐剂作用进行清除。通过对菌落总数、霉菌和酵母菌数方法学验证以及控制菌检查方法学验证对比了常规法与薄膜过滤法的效果。结果表明,常规法对照用阳性菌金黄色葡萄球菌、枯草芽孢杆菌、白色念珠菌和黑曲霉的回收率小于70%,而薄膜过滤法的回收率均大于90%。

A test method for inspection of microorganism in toilet water

A test method for inspection of microorganism in toilet water was established.Physiological saline containing 0.1% mass fraction of Tween 80 was used as flushing liquid,and membrane filtration was adopted for removal of antiseptic function containing in the toilet water sample.Antiseptic action removal efficacy of the membrane filtration method was compared with that of the conventional method via the methodology of total number of bacterial colony,total number of molds and total number of saccharomycete as well as methodology of control bacteria inspection.Results showed that recovery of Staphylococcus aureus,Bacillus subtilis,Candida albicans and Aspergillus niger of the conventional method is lower than 70%,while that of the membrane filtration method is higher than 90%.