Electrotransformation of Clostridium paraputrificum M-21 with some plasmids |
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Authors: | Sakka Kazuo Kawase Mamiko Baba Daisuke Morimoto Kenji Karita Shuichi Kimura Tetsuya Ohmiya Kunio |
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Affiliation: | Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu 514-8507, Japan. sakka@bio.mie-u.ac.jp |
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Abstract: | Clostridium paraputrificum M-21 was transformed with several shuttle plasmids constructed for Clostridium acetobutylicum-Escherichia coli and Clostridium perfringens-E. coli by electroporation. The Clostridium stercorarium xylanase gene xyn10B was successfully expressed in C. paraputrificum M-21 and the expressed protein did not suffer from proteolysis by host protease(s). This system will provide us with a genetic tool for genetic and metabolic engineering of this bacterium. |
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