Autotoxic Compounds from Fresh Alfalfa Leaf Extracts: Identification and Biological Activity |
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Authors: | Ill-Min Chung David Seigler Darrell A Miller Suk-Hun Kyung |
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Affiliation: | (1) Department of Crop Science, KonKuK University Seoul, South Korea, 143-701;(2) Department of Plant Biology, University of Illinois, Urbana-Champaign, Illinois, 61801;(3) Department of Crop Science, University of Illinois, Urbana-Champaign, Illinois, 61801;(4) Department of Applied Biology and Chemistry, Konkuk University, Seoul, South Korea, 143-701 |
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Abstract: | Many investigators have attempted to identify the allelochemicals in alfalfa (Medicago sativa), that cause autotoxicity. The autotoxic compounds from fresh alfalfa leaves were separated and quantified, and their biological activity was determined. Chemical separation procedures involved an 80% methanol extract of fresh alfalfa leaves, treatment with activated charcoal, microcrystalline cellulose thin-layer chromatography (MCTLC), and finally separation by Sephadex LH-20 column chromatography. The various fractions were examined further by high-performance liquid chromatography (HPLC). Preliminary identification by HPLC analysis resulted in peaks with retention times close to those of chlorogenic (m/z = 354) and salicylic acid (m/z = 138) standards, and these compounds were confirmed with GC-MS. Several other peaks remain unidentified. Chlorogenic acid occurs in relatively large amounts (0.39 mg/g) in alfalfa aqueous extracts as compared to salicylic acid (0.03 mg/g), and bioassays suggest that chlorogenic acid is involved in alfalfa autotoxicity. |
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Keywords: | Alfalfa extract autotoxicity bioassay chlorogenic acid salicylic acid HPLC GC-MS |
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