Production of D-p-Hydroxyphenylglycine From DL-5-p-Hydroxyphenyl Hydantoin by Immobilized Pseudomonas Putida Cells in a Hollow Fiber Memgrane Bioreactor

1 INTRODUCTION D-p-Hydroxyphenylglycine(D-pHPG) is one of the valuable intermediates in the production of semisynthetic β -lactam antibiotics. It can be used for the production of semisynthetic penicillin and cephalosporin. With the increasing demand for semisynthetic antibiotics, the production of D-pHPG from DL-5-p-hydroxyphenyl hydantoin (DL-pHPH) has received more and more attention. Compared with the chemo-enzymatic route developed by Yamada et al. [1,2], the direct enzymatic production of D-pHPG has advantages of low environmental pollution, less equipment needed and mild reaction conditions.