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Detection of cytochrome P450 substrates by using a small-molecule droplet array on an NADH-immobilized solid surface |
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| Authors: | Takayama Hiroshi Takahashi Shunji Moriya Takashi Osada Hiroyuki Iwabuchi Yoshiharu Kanoh Naoki |
| Affiliation: | Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan. |
| Abstract: | Seeing below the surface: A small-molecule droplet array platform on an NADH-immobilized solid surface and a biotinylated acetophenone derivative were developed to identify the substrate candidates for soluble P450 enzymes of interest. This methodology is thought to be easily applicable to other class I P450 systems, including those that use NADPH as cofactor. |
| Keywords: | cytochromes immobilization microarrays nicotinamide adenine dinucleotide substrate screening |
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