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The impact of different hop compounds on the growth of selected beer spoilage bacteria in beer
Authors:Maximilian Michel  Sandro Cocuzza  Martin Biendl  Frank Peifer  Sebastian Hans  Yvonne Methner  Friedrich Pehl  Werner Back  Fritz Jacob  Mathias Hutzler
Affiliation:1. Research Center Weihenstephan for Brewing and Food Quality, TU München, Alte Akademie 3, Freising, 85354 Germany;2. Hopsteiner, Simon H. Steiner, Hopfen GmbH, Auhofstrasse 18, Mainburg, 84048 Germany;3. Neumarkter Lammsbräu Gebr. Ehrnsperger KG, Amberger Str. 1, Neumarkt, 92318 Germany;4. TU München, Wissenschaftszentrum Weihenstephan, Freising, 85354 Germany
Abstract:Beer spoiling lactic acid bacteria are a major reason for quality complaints in breweries around the world. Spoilage by a variety of these bacteria can result in haze, sediment, slime, off-flavours and acidity. As these bacteria occur frequently in the brewing environment, using certain hop products that inhibit the growth of these spoilers could be a solution to prevent problems. To investigate the impact of seven different hop compounds (α-acids, iso-α-acids, tetrahydro-iso-α-acids, rho-iso-α-acids, xanthohumol, iso-xanthohumol and humulinones) on the growth of six major beer spoilage bacteria (Lactobacillus brevis. L. backi, L. coryniformis, L. lindneri, L. buchneri, Pediococcus damnosous), two concentrations (10 and 25 mg/L) of each hop substance were added to unhopped beer. The potential growth of the spoilage bacteria was investigated over 56 consecutive days. A comparison of the results shows a strong inhibition of growth of all spoilage bacteria at 25 mg/L of tetrahydro-iso-α-acids closely followed by α-acids as the second most inhibitory substance. The results showed a high resistance of L. brevis to all hop compounds as well as an inhibition of L. coryniformis and L. buchneri at low concentrations of most hop components. In comparison with the control sample, L. lindneri showed increased growth in the presence of some hop compounds (rho-iso-α-acids, xanthohumol, iso-xanthohumol, humulinones). © 2020 The Authors. Journal of the Institute of Brewing published by John Wiley & Sons Ltd on behalf of The Institute of Brewing & Distilling
Keywords:microbiological stability  shelf life  bittering hop acids  beer spoilage bacteria
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