Optimisation of a polymer membrane used in optical oxygen sensing |
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| Affiliation: | 1. Department of Physics, University of Brasov, 2200 Brasov, Romania;2. Institut für Chemo und Biosensorik, 48149 Münster, Germany;1. Key Laboratory of Novel Thin Film Solar Cells, Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, 230088, PR China;2. University of Science and Technology of China, Hefei, Anhui, 230026, PR China;3. NAAM Research Group, Department of Mathematics, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia;4. Department of Mathematics, Quaid-I-Azam University, Islamabad, 44000, Pakistan;5. Beijing Key Laboratory of Novel Thin Film Solar Cells, School of Renewable Energy, North China Electric Power University, No 2, Beinong Rd, Changping, Beijing, 102206, PR China;6. Hubei Key Laboratory of Ferro & Piezoelectric Materials and Devices, Faculty of Physics and Electronic Sciences, Hubei University, Wuhan, 430062, PR China;1. Department of Neurology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California;4. Department of Pathology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California;6. Department of Neurosurgery, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California;7. Department of Radiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, California;2. Department of Neurology, School of Medicine at University of California, Irvine, Irvine, California;3. School of Public Health, Xinxiang Medical University, Xinxiang, China, and Curtin University, Perth, Western Australia, Australia;11. Department of Neurology, Columbia University, New York, New York;5. Kaiser Permanente Southern California, Los Angeles, California;1. Department of Advanced Organic Materials Science and Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea;2. Department of Medical and Biological Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea;3. Department of Molecular Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea;4. Department of Bio-fibers and materials Science, Kyungpook National University, Daegu 702-701, Republic of Korea;1. Laboratório de Materiais Híbridos, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, Brazil;2. Laboratório de Cristalografia, Instituto de Física, Universidade de São Paulo, SP, Brazil |
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| Abstract: | A method for detection of viable cells utilises a sensor based on the optical measurement of oxygen consuming by cells. Changes in the oxygen level were measured via quenching of the fluorescence of an oxygen-sensitive fluorophor (Ru(dpp)3Cl2). The fluorescence lifetime changing was measured in accord with Stern–Volmer equation, using a phase-shift method. The fluorophor was embedded into a polysulfone membrane that is in contact with the cell medium. The sensitivity of oxygen sensor depends on behaviour of polysulfone membrane. Manufacturing method, type of polysulfone and concentration of fluorophor can also change this behaviour. These parameters were explored to obtain the optimum analytical performance, and the optimum sensitive membrane was chosen for 3 mmol/l concentration of fluorophor, when a linear plot was obtained with R=0.99987 for a sensitivity of 12.11±0.11 mV/% O2 (n=5). |
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