Thick film sensors based on laccases from different sources immobilized in polyaniline matrix |
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| Affiliation: | 1. Biochemistry Department, Faculty of Science, Ege University, Bornova, Izmir 35100, Turkey;2. ENEA C.R. Casaccia, Via Anguillarese 301-I-00060-Santa Maria di Galeria, Rome, Italy;1. Kaliningrad State Technical University, Kaliningrad, Russia;2. Immanuel Kant Baltic Federal University, Kaliningrad, Russia;1. Zhejiang Provincial Key Laboratory of Fiber Materials and Manufacturing Technology, Zhejiang Sci-Tech University, Hangzhou, 310018, China;2. Department of Materials Science & Engineering, College of Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, United States;3. Zhejiang Kertice Hi-Tech Fluor-Material Co., LTD, Huzhou, 313000, China;4. School of Textile Science and Engineering, Xi’an Polytechnic University, Xi’an, 710048, China;1. Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand;2. Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok 10900, Thailand;3. School of Engineering and Science, Tecnologico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, NL CP 64849, Mexico;4. Faculty of Science and Technology, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK;1. Department of Chemical Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea;2. Department of Food Science & Biotechnology, Shin-Ansan University, Ansan 15435, Republic of Korea |
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| Abstract: | Thick film electrode based biosensors containing Trametes versicolor (TvL) and Aspergillus niger (AnL) laccases and Agaricus bisporus tissues (AbT) were developed for the determination of phenolic compounds and the measurement was based on oxygen consumption in relation to analyte oxidation. The electrodeposited organic polymer; polyaniline was used as a matrix for the immobilization in the preparation of thick film sensors. The systems were calibrated for different phenolic substances. A linearity was obtained in concentration range between 0.4 and 6.0 μM phenol, 0.2 and 1.0 μM catechol, 2.0 and 20.0 μM l-DOPA for TvL based biosensor; for AnL based enzyme electrode 0.4 and 4.0 μM phenol, 0.4 and 15 μM catechol, 0.4 and 6.0 μM l-DOPA; for AbT electrode 1.0 and 10 μM phenol, 0.4 and 1.6 μM catechol, 1.0 and 10 μM l-DOPA, respectively, in the response time of 300 s. Furthermore, as well as sample application and accuracy, optimum pH, temperature and thermal stabilities of the proposed systems were also detected. |
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