| 西藏Kefir粒中菌相的初步研究 |
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| 引用本文： | 许女,王艳萍,习傲登,张玢. 西藏Kefir粒中菌相的初步研究[J]. 中国酿造, 2011, 0(10) |
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| 作者姓名： | 许女 王艳萍 习傲登 张玢 |
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| 作者单位： | 1. 山西农业大学食品科学与工程学院,山西太谷,030801
2. 教育部天津市食品营养与安全重点实验室,天津科技大学食品工程与生物技术学院,天津300457 |
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| 摘 要： | 利用扫描电镜、传统菌种分离及PCR-DGGE 3种技术相结合,对Kefir粒的菌相作了初步研究,试验结果显示,Kefir粒内层菌体密度较大,以短杆菌为主,有部分长杆菌,还有部分椭圆形酵母；对其中的乳酸菌和酵母菌进行了分离,共得到9株乳球菌、38株乳杆菌和9株酵母菌,描述了各菌株的细胞、菌落形态,研究了其牛乳发酵特性和半乳糖苷酶活性,并且对Kefir乳发酵过程中的菌数变化做了动态监测.
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| 关 键 词： | 开菲尔 菌相 变性梯度凝胶电泳 |
Microbial populations analysis of Tibet Kefir |
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| XU Nv,WANG Yanping,XI Aodeng,ZHANG Bin. Microbial populations analysis of Tibet Kefir[J]. China Brewing, 2011, 0(10) |
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| Authors: | XU Nv WANG Yanping XI Aodeng ZHANG Bin |
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| Affiliation: | XU Nv1,WANG Yanping2,XI Aodeng2,ZHANG Bin2(1.Food Science and Engineering College,Shanxi Agricultural University,Taigu 030801,2.Key Laboratory of Food Nutrition and Safety,Ministry of Education,Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | The microorgnisms in Kefir grains were preliminarily studied by combination of scanning electronic microscope,traditional bacteria plating isolating technique and PCR-DGGE.The results showed that the bacteria density in the inner layer of Kefir grains is bigger,which mainly composed of Bacillus brevis,some long bacillus and some oval-shaped yeast.Total 9 Lactococcus strains,38 Lactobacillus strains and 9 yeast strains were isolated,cellular and colonial morphology of various strains were described,as well.F... |
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| Keywords: | Kefir microbial populations DGGE |
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