| 低温冷冻对皮肤组织桥粒芯糖蛋白1表达影响的实验研究 |
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| 引用本文： | 贾晓明,李东杰,马彩虹. 低温冷冻对皮肤组织桥粒芯糖蛋白1表达影响的实验研究[J]. 压电与声光, 2010, 0(1): 53-54,57 |
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| 作者姓名： | 贾晓明 李东杰 马彩虹 |
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| 作者单位： | 解放军总医院第一附属医院全军烧伤研究所,北京100048 |
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| 基金项目： | 国家自然科学基金项目 (30070298,30672177);;首都医学发展科研基金项目 (2005-3087)~~ |
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| 摘 要： | 目的观察桥粒芯糖蛋白1（Desmogleinl，Dsg1）在不同温度保存皮肤组织中的分布，以探讨其与皮肤冷冻损伤的关系。方法用Dsg1抗体对5种保存条件下的皮肤组织作免疫组化染色，采用计算机图像定量分析方法测定表皮组织中的含量，并用H．E染色观察皮肤的组织结构变化。结果与新鲜皮片组相比，-196℃条件下皮肤组织结构保存较好，Dsg1含量变化不明显，而4℃、-20℃、-80℃保存皮肤组织结构破坏明显，含量亦明显下降。结论皮肤低温保存冷冻损伤可能与桥粒跨膜蛋白Dsg1破坏有关。
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| 关 键 词： | 皮肤 桥粒芯糖蛋白1 低温保存 |
Expression of desmoglein 1 in cryopreserved human skin |
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| JIA Xiao-ming,LI Dong-jie,MA Cai-hongBurning Institute,Clinical Division,Chinese PLA General Hospital,Beijing,China. Expression of desmoglein 1 in cryopreserved human skin[J]. Piezoelectrics & Acoustooptics, 2010, 0(1): 53-54,57 |
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| Authors: | JIA Xiao-ming LI Dong-jie MA Cai-hongBurning Institute Clinical Division Chinese PLA General Hospital Beijing China |
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| Affiliation: | JIA Xiao-ming,LI Dong-jie,MA Cai-hongBurning Institute,304 Clinical Division,Chinese PLA General Hospital,Beijing 100048,China |
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| Abstract: | Objective To observe the distribution of desmoglein 1(Dsg 1) in cryopreserved human skin and to investigate its relation with skin cryoinjury. Methods Skin tissues cryopreserved under 5 different conditions were stained with immunohistochemistry using antibodies of dsg 1. Computer image quantitative analysis was used to measure DSG 1 level in epidermal tissues. Changes in structure of epidermal tissue were observed with H&E staining. Results Compared to the normal human skin, no significant change was found... |
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| Keywords: | Skin Desmoglein1 Cryopreservation |
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