| 乙型肝炎病毒前-S2蛋白反式激活蛋白1基因的克隆化研究 |
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| 引用本文： | 纪冬,刘妍,韩萍,陈国凤,辛绍杰.乙型肝炎病毒前-S2蛋白反式激活蛋白1基因的克隆化研究[J].压电与声光,2010(1):55-57. |
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| 作者姓名： | 纪冬 刘妍 韩萍 陈国凤 辛绍杰 |
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| 作者单位： | [1]解放军军医进修学院,北京100853 [2]解放军第302医院,北京100039 |
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| 基金项目： | 国家自然科学基金(30700713);;军队“十一五”课题(06MA361)~~ |
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| 摘 要： | 目的应用基因表达谱芯片技术及生物信息学技术筛选并克隆乙型肝炎病毒（HBV）前-S2蛋白反式激活新型靶基因，进一步阐明HBV感染相关性疾病的发病机制。方法以HBV前-S2蛋白表达质粒pcDNA3．1（-）-PreS2转染HepG2细胞，以空载体pcDNA3．1（-）为平行对照，提取总RNA进行基因表达谱芯片分析。并克隆HBV前-S2反式激活作用的新的靶基因。结果获得该新基因的全长序列，并测序证实，因其可以被前-S2蛋白反式激活，故命名为前S2反式激活蛋白1（PS2TP1），已在GenBank中注册，注册号：AY561706。PS2TP1基因的编码序列全长为1113个核苷酸（nt），编码产物由370个氨基酸残基（aa）组成。结论HBV前-S2蛋白具有反式激活功能，调节宿主细胞某些基因的表达，从而改变宿主正常的应答水平，引起病变。
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| 关 键 词： | 嗜肝DNA病毒属 蛋白 反式激活遗传学 基因 克隆，分子 |
Cloning of human gene 1 transactivated by pre-S2 protein of hepatitis B virus |
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| JI Dong,LIU Yan,HAN Ping,CHEN Guo-feng,XIN Shao-jieMilitary Postgraduate Medical School,Beijing,Chinese PLA Hospital,Beijing,China.Cloning of human gene 1 transactivated by pre-S2 protein of hepatitis B virus[J].Piezoelectrics & Acoustooptics,2010(1):55-57. |
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| Authors: | JI Dong LIU Yan HAN Ping CHEN Guo-feng XIN Shao-jieMilitary Postgraduate Medical School Beijing Chinese PLA Hospital Beijing China |
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| Affiliation: | JI Dong1,2,LIU Yan2,HAN Ping1,CHEN Guo-feng1,XIN Shao-jie21Military Postgraduate Medical School,Beijing 100853,2Chinese PLA302 Hospital,Beijing 100039,China |
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| Abstract: | ObjectiveTo screen and clone the target genes transactivated by hepatitis B virus (HBV) Pre-S2 protein in order to pave away for elucidating the pathogenesis of HBVinfection.MethodsHBVPre-S2 coding DNAfragment was amplified by polymerase chainreaction (PCR) using G3767 plasmid containing the full length of HBVgenome as a template. An expressive vector of pcDNA3.1 Pre-S2 was constructed with routine molecular biological methods. HepG2 cells were transfected with pcDNA3.1 (-) and pcDNA3.1 Pre-S2, respectively... |
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| Keywords: | Orthohepadnavirus Protein Trans-Activation Genetics Genes Cloning Molecular |
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