| 人DC-SIGN真核表达载体的构建及表达 |
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| 引用本文： | 王宇,阎瑾琦,余云舟,贾锐,张亮,张巍,李韧,高明,于继云. 人DC-SIGN真核表达载体的构建及表达[J]. 压电与声光, 2010, 0(1): 45-47 |
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| 作者姓名： | 王宇 阎瑾琦 余云舟 贾锐 张亮 张巍 李韧 高明 于继云 |
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| 作者单位： | [1]军事医学科学院基础医学研究所,北京100850 [2]武警总医院肿瘤中心,北京100039 |
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| 基金项目： | 国家“863”基金项目 (2006AA02A237, 2007AA02Z451);;国家自然科学基金项目 (30772002)~~ |
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| 摘 要： | 目的构建含有FLAG标签的人DC—SIGN真核表达载体，并在人胚肾293T细胞中进行表达。方法通过PCR方法获得含有FLAG标签的人DC—SIGN分子基因，将其插入到真核表达载体pIRES—neo中。构建的重组质粒pIRES—neO—FLAG—DC—SIGN转染293T细胞，利用流式细胞仪、免疫荧光及WesternBlot检测其表达情况。结果含FLAG标签的人DC—SIGN基因测序正确，PCR和酶切鉴定证明FLAG—DC—SIGN基因已成功连入真核表达载体pIRES—neo中；检测结果显示重组质粒pIRES—neo—FLAG—DC—SIGN在293T细胞中得到表达。结论成功构建了重组质粒pIRES—neo—FLAG—DC—SIGN，且在293T细胞中能有效表达，为后续DC靶向性疫苗研究奠定了基础。
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| 关 键 词： | DC-SIGN 基因表达 293T细胞 |
Construction of human DC-SIGN eukaryotic expression vector and its expression |
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| WANG Yu,YAN Jin-qi,YU Yun-zhou,JIA Rui,ZHANG Liang,ZHANG Wei,LI Ren,GAO Ming,YU Ji-yunInstitute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing,Chin,Cancer Center of Chinese People's Armed Police Forces General Hospital,Beijing. Construction of human DC-SIGN eukaryotic expression vector and its expression[J]. Piezoelectrics & Acoustooptics, 2010, 0(1): 45-47 |
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| Authors: | WANG Yu YAN Jin-qi YU Yun-zhou JIA Rui ZHANG Liang ZHANG Wei LI Ren GAO Ming YU Ji-yunInstitute of Basic Medical Sciences Academy of Military Medical Sciences Beijing Chin Cancer Center of Chinese People's Armed Police Forces General Hospital Beijing |
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| Affiliation: | WANG Yu1,YAN Jin-qi1,YU Yun-zhou1,JIA Rui1,ZHANG Liang1,ZHANG Wei1,LI Ren2,GAO Ming1,YU Ji-yun11Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850,China,2Cancer Center of Chinese People's Armed Police Forces General Hospital,Beijing 100039 |
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| Abstract: | Objective To construct a eukaryotic expression plasmid of human DC-SIGN gene containing a FLAG tag and detect its expression in eukaryotic cell 293T. Methods Human DC-SIGN gene containing FALG tag was constructed by PCR and inserted into a eukaryotic expression vector pIRES-neo. The recombinant plasmid pIRES-neo-FLAG-DC-SIGN was transfected to the 293T cells, and its expression was detected by FACS, IMF and Western blot. Results The gene sequence of human DC-SIGN containing FLAG was consistent with that of ... |
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| Keywords: | DC-SIGN Gene Expression 293T Cell |
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