ZnO纳米材料抗凝血性能的初步评价

采用体外动态凝血时间实验评价了ZnO纳米材料的抗凝血性能. 纳米材料的平均粒度在10~30 nm. 实验分为对照组(抗凝全血1 ml)、无纳米组(抗凝全血1 ml，包括搅拌血浆0.4 ml)、ZnO纳米材料组(抗凝全血1 ml，包括搅拌血浆0.4 ml，加入ZnO纳米材料1 mg)和Fe纳米材料组(抗凝全血1 ml，包括搅拌血浆0.4 ml，加入Fe纳米材料1 mg). 每组样品分别在5, 15, 30及45 min测定光密度值，计算结果表明，ZnO纳米材料的抗凝血率在45 min时仍保持在70%，而对照组和Fe纳米材料组的抗凝血率在15 min时已降至30%. 实验表明ZnO纳米材料具有良好的抗凝血作用.

Preliminary Evaluation of the Thromboresistance of ZnO Nanoparticles

Evaluation of antithrombogenicity of ZnO nanoparticles was carried out based on the measurements of in vitro kinetic clotting time. The average size of the ZnO nanoparticles was 10~30 nm. The research was divided into the control group (1 ml of whole blood), the non-nanoparticles group (1 ml of whole blood, including 0.4 ml of the stirred plasma), the ZnO group (1 ml of whole blood, including 0.4 ml of the stirred plasma and 1 mg of ZnO nanoparticles) and the Fe group (1 ml of whole blood, including 0.4 ml of the stirred plasma and 1 mg of Fe nanoparticles). Measurements of clotting time of every group were carried out at 5, 15, 30 and 45 min. The calculated results revealed that the thromboresistant radio was remained at 70% in 45 min in the ZnO nanoparticles group, while the radio dropped to 30% in 15 min in the control group, the non-nanoparticles group and the Fe group. The results predict that the ZnO nanoparticles have excellent thromboresistant property.