五个产地荔枝核中药饮片抗氧化活性研究

为了比较五个产地荔枝核中药饮片的体外抗氧化活性,分别采用DPPH法、ABTS法和总抗氧化能力测试法（T-AOC）对5个产地荔枝核的90%乙醇提取物进行抗氧化活性测定,同时对其中的活性成分包括多酚、黄酮和皂苷及其含量与抗氧化活性的相关性进行了初步分析。结果表明:广东惠东荔枝核中多酚和黄酮含量最高,其次为阳西荔枝核,广西灵山荔枝核多酚和黄酮含量均最低;阳西荔枝核中皂苷含量最高,其他产地皂苷含量从高到低依次为广东惠东、从化、东莞和广西灵山。各产地荔枝核提取液对DPPH自由基的清除率差异均不显著,而对ABTS+自由基的清除率差异显著,其中广东阳西荔枝核的清除能力最强,总抗氧化能力测定结果表明广东从化荔枝核抗氧化能力最强,广西灵山荔枝核最弱。活性物质与抗氧化活性相关性分析表明多酚、黄酮和皂苷均与抗氧化活性呈现一定的正相关。 

Study on the antioxidant activity of Chinese herbal medicine prepared from litchi (Litchi chinensis sonn) seed in five producing areas

The antioxidant activity of litchi seed from five producing areas was estimated in vitro models using1, 1-Diphenyl-2-picrylhydrazyl (DPPH) method, 2, 2'-Azinobis- (3-ethylbenzthiazoline-6-sulphonate) (ABTS) method and total antioxidant capacity (T-AOC) assay method. The content of phenolics, flavonoids and saponins was also determined, and the correlation analysis was carried out between the active constituent and antioxidant capacity. The results showed that the content of phenolics and flavonoids in the litchi seed produced in Huidong was the highest among the five places followed by that produced in Yangxi with the lowest content produced in Lingshan. In addition, the Yangxi litchi seed had the highest level of saponin followed by that of Huidong, Conghua, Dongguan and Lingshan in sequence. Furthermore, no significant difference was found about the DPPH radical scavenging capacity among litchi seeds from the five places, while the ABTS+radical scavenging capacity was significantly different and the Yangxi litchi seed had the strongest scavenging ability.The total antioxidant capacity of litchi seed in Conghua was the highest and that was lowest in Lingshan litchi.Moreover, certain positive correlation was found between the antioxidant activity and phenolics, flavonoids and saponins content in litchi seed.